Autumn Royal and Ribier Grape Juice Extracts Reduced Viability and Metastatic Potential of Colon Cancer Cells

Manuel Valenzuela; Lorena Bastias; Iván Montenegro; Enrique Werner; Alejandro Madrid; Patricio Godoy; Mario Párraga; Joan Villena

doi:10.1155/2018/2517080

Autumn Royal and Ribier Grape Juice Extracts Reduced Viability and Metastatic Potential of Colon Cancer Cells

Manuel Valenzuela
, Lorena Bastias
, Iván Montenegro
, Enrique Werner
, Alejandro Madrid
, Patricio Godoy
, Mario Párraga
, Joan Villena

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Antioxidants are known to be beneficial to health. This paper evaluates the potential chemopreventive and anticancer properties of phenolic compounds present in grape juice extracts (GJE) from Autumn Royal and Ribier varieties. The effects of these GJE on viability (SRB day assay) and metastatic potential (migration and invasion parameters) of colon cancer cell lines HT-29 and SW-480 were evaluated. The effects of GJE on two matrix metalloproteinase gene expressions (MMP2 and MMP9) were also evaluated via qRT-PCR. In the former, GJE reduced cell viability in both cell lines in a dose-dependent manner. GJE treatment also reduced cell migration and invasion. Moreover, MMP-2 and MMP-9 gene expression diminished depending on extract and on cell type. Conclusions. These results provide novel information concerning anticancer properties of selected GJE by revealing selective cytotoxicity and the ability to reduce invasiveness of colon cancer cells.

Original language	English
Article number	2517080
Journal	Evidence-based Complementary and Alternative Medicine
Volume	2018
DOIs	https://doi.org/10.1155/2018/2517080
State	Published - 2018

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title = "Autumn Royal and Ribier Grape Juice Extracts Reduced Viability and Metastatic Potential of Colon Cancer Cells",

abstract = "Antioxidants are known to be beneficial to health. This paper evaluates the potential chemopreventive and anticancer properties of phenolic compounds present in grape juice extracts (GJE) from Autumn Royal and Ribier varieties. The effects of these GJE on viability (SRB day assay) and metastatic potential (migration and invasion parameters) of colon cancer cell lines HT-29 and SW-480 were evaluated. The effects of GJE on two matrix metalloproteinase gene expressions (MMP2 and MMP9) were also evaluated via qRT-PCR. In the former, GJE reduced cell viability in both cell lines in a dose-dependent manner. GJE treatment also reduced cell migration and invasion. Moreover, MMP-2 and MMP-9 gene expression diminished depending on extract and on cell type. Conclusions. These results provide novel information concerning anticancer properties of selected GJE by revealing selective cytotoxicity and the ability to reduce invasiveness of colon cancer cells.",

author = "Manuel Valenzuela and Lorena Bastias and Iv{\'a}n Montenegro and Enrique Werner and Alejandro Madrid and Patricio Godoy and Mario P{\'a}rraga and Joan Villena",

note = "Publisher Copyright: {\textcopyright} 2018 Manuel Valenzuela et al.",

year = "2018",

doi = "10.1155/2018/2517080",

language = "English",

volume = "2018",

journal = "Evidence-based Complementary and Alternative Medicine",

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TY - JOUR

T1 - Autumn Royal and Ribier Grape Juice Extracts Reduced Viability and Metastatic Potential of Colon Cancer Cells

AU - Valenzuela, Manuel

AU - Bastias, Lorena

AU - Montenegro, Iván

AU - Werner, Enrique

AU - Madrid, Alejandro

AU - Godoy, Patricio

AU - Párraga, Mario

AU - Villena, Joan

PY - 2018

Y1 - 2018

N2 - Antioxidants are known to be beneficial to health. This paper evaluates the potential chemopreventive and anticancer properties of phenolic compounds present in grape juice extracts (GJE) from Autumn Royal and Ribier varieties. The effects of these GJE on viability (SRB day assay) and metastatic potential (migration and invasion parameters) of colon cancer cell lines HT-29 and SW-480 were evaluated. The effects of GJE on two matrix metalloproteinase gene expressions (MMP2 and MMP9) were also evaluated via qRT-PCR. In the former, GJE reduced cell viability in both cell lines in a dose-dependent manner. GJE treatment also reduced cell migration and invasion. Moreover, MMP-2 and MMP-9 gene expression diminished depending on extract and on cell type. Conclusions. These results provide novel information concerning anticancer properties of selected GJE by revealing selective cytotoxicity and the ability to reduce invasiveness of colon cancer cells.

AB - Antioxidants are known to be beneficial to health. This paper evaluates the potential chemopreventive and anticancer properties of phenolic compounds present in grape juice extracts (GJE) from Autumn Royal and Ribier varieties. The effects of these GJE on viability (SRB day assay) and metastatic potential (migration and invasion parameters) of colon cancer cell lines HT-29 and SW-480 were evaluated. The effects of GJE on two matrix metalloproteinase gene expressions (MMP2 and MMP9) were also evaluated via qRT-PCR. In the former, GJE reduced cell viability in both cell lines in a dose-dependent manner. GJE treatment also reduced cell migration and invasion. Moreover, MMP-2 and MMP-9 gene expression diminished depending on extract and on cell type. Conclusions. These results provide novel information concerning anticancer properties of selected GJE by revealing selective cytotoxicity and the ability to reduce invasiveness of colon cancer cells.

UR - https://www.scopus.com/pages/publications/85045896719

U2 - 10.1155/2018/2517080

DO - 10.1155/2018/2517080

M3 - Article

AN - SCOPUS:85045896719

SN - 1741-427X

VL - 2018

JO - Evidence-based Complementary and Alternative Medicine

JF - Evidence-based Complementary and Alternative Medicine

M1 - 2517080

ER -

Autumn Royal and Ribier Grape Juice Extracts Reduced Viability and Metastatic Potential of Colon Cancer Cells

Abstract

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